TET3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43151

Core Product Specifications and Parameters

Biological Background: TET3 Function and Localization

• TET3 is a dioxygenase that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and further oxidized derivatives, critical for epigenetic chromatin reprogramming in the zygote after fertilization. Related references: PMID:31928709
• Selectively binds to promoter regions of target genes, thereby regulating the expression of numerous developmental genes. Related references: PMID:23217707
• Mediates paternal pronucleus DNA demethylation in zygotes while maternal chromatin is protected by DPPA3/PGC7, ensuring asymmetric reprogramming.
• Recruits O-GlcNAc transferase (OGT) to CpG-rich transcription start sites, promoting histone H2B GlcNAcylation and epigenetic regulation. Related references: PMID:23353889
• Preferentially binds CpG dinucleotides over hemimethylated or CpH motifs, underscoring its specificity. Related references: PMID:29276034
• Localizes to the nucleus, cytoplasm, and chromosomes, consistent with its roles in DNA modification and transcriptional regulation.
• Expressed in colon, muscle, adrenal gland, and peripheral blood lymphocytes, with potential tissue-specific regulatory functions.
• Features a zinc-finger domain, alternative splicing, and has been associated with disease variants in intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1551-1650) of human TET3, which is conserved among human, mouse, and rat, supporting the observed cross-reactivity.
• For WB, consider the high molecular weight (~194 kDa); use low-percentage SDS-PAGE gels and optimized transfer conditions for large proteins.
• For IHC-P and IF/ICC, validate staining in known TET3-positive tissues (e.g., colon or muscle) and include appropriate controls to confirm specificity.
• The polyclonal antibody may exhibit robust signal, but lot-to-lot variability is possible; we recommend titrating and validating each new lot in your system.
• For ELISA applications, pair with a suitable detection antibody and test serial dilutions of recombinant protein or cell lysates to establish optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials and technical services, supporting your journey from concept to clinic. This TET3 rabbit polyclonal antibody is ideal for epigenetics, DNA methylation, and developmental biology research. Beyond this reagent, we offer validated antibody pairs, optimized mono/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your diagnostic assay development.