TET3 Rabbit Monoclonal Antibody for WB and ELISA - Q8BG87

Core Product Specifications and Parameters

Biological Background: TET3 Function and Localization

• TET3 is a methylcytosine dioxygenase that catalyzes the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), initiating active DNA demethylation.
• Plays a critical role in epigenetic reprogramming of the paternal pronucleus in zygotes after fertilization, while maternal pronucleus demethylation is blocked by DPPA3/PGC7.
• Binds preferentially to CpG-rich promoter regions of target genes, contributing to the regulation of developmental gene expression.
• Also recruits O-GlcNAc transferase (OGT) to CpG-rich transcription start sites, promoting histone H2B GlcNAcylation and influencing chromatin structure (by similarity).
• Contains zinc-finger domains and utilizes iron and 2-oxoglutarate for its dioxygenase activity, highlighting its metal-dependent catalytic mechanism.
• Localizes primarily to the nucleus and chromosomes, with additional presence in the cytoplasm, consistent with its role in chromatin modification.
• Highly expressed in oocytes (germinal vesicle and MII stages) and early embryos, underscoring its maternal-effect function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-285) of mouse TET3, which may target an epitope within a functional domain; consider its relevance for detecting full-length and truncation variants.
• For Western blot, the predicted molecular weight is approximately 195kDa; validate band specificity using appropriate controls and consider potential post-translational modifications.
• In ELISA applications, test with recombinant protein or cell lysates to confirm linearity and sensitivity in your specific experimental context.
• Since the antibody shows reactivity with mouse samples, cross-test in related species if needed, but verify cross-reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this TET3 antibody for research into epigenetic mechanisms and developmental biology. In addition to validated monoclonal antibodies like this anti-TET3 clone, we provide antibody pairs, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to support your diagnostic and research workflows.