TEAD1 Rabbit mAb for WB, IP, ELISA, ChIP, CUT&Tag - P28347

Core Product Specifications and Parameters

Biological Background: TEAD1 Function and Localization

• TEAD1 (Transcriptional enhancer factor TEF-1), also known as NTEF-1, TCF-13, and TEAD-1, is a member of the TEA domain transcription factor family.
• As a key downstream effector of the Hippo signaling pathway, TEAD1 mediates gene expression driven by YAP1 and WWTR1/TAZ co-activators, controlling cell proliferation, migration, and epithelial–mesenchymal transition (EMT).
• TEAD1 binds specifically to the SPH and GT-IIC enhanson motifs (5'-GTGGAATGT-3') and the M-CAT motif, activating transcription in a cell‑type‑specific manner, likely requiring a limiting transcriptional intermediary factor.
• It plays an essential role in cardiac development, where it regulates gene programs critical for heart morphogenesis.
• The protein localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcriptional regulator.
• TEAD1 expression is highest in skeletal muscle, with lower levels in pancreas, placenta, and heart.
• Post-translational modifications include phosphorylation and acetylation, which may modulate its activity, DNA binding, or interactions with co-factors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human TEAD1; antibody reactivity may be influenced by post-translational modifications or interaction partners that mask this epitope.
• Because TEAD1 is conserved across human, mouse, and rat, this antibody is suitable for cross-species studies, but consider verifying reactivity in your specific sample matrix.
• For Western blotting, a band near 48 kDa is expected; nuclear enrichment protocols may improve signal intensity.
• The antibody’s performance in ChIP and CUT&Tag suggests it can recognize endogenous protein in chromatin context; optimization of cross-linking and shearing conditions is recommended.
• When using IP or ELISA, include appropriate negative controls to account for non-specific binding of rabbit monoclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing platform for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For TEAD1 and Hippo pathway research, we offer a range of ready-to-use reagents and can assist in sourcing rare-target raw materials. Our team supports you from concept to clinic, ensuring reliable supply and expert technical guidance.