TCEB2 Rabbit pAb - Q15370

Core Product Specifications and Parameters

Biological Background: TCEB2 Function and Localization

• TCEB2 encodes Elongin-B (Elongin 18 kDa subunit, SIII p18), a subunit of the transcription elongation factor SIII (elongin) and a core component of multiple cullin-2/5-RING E3 ubiquitin ligase complexes (ECS). The protein is also known as RNA polymerase II transcription factor SIII subunit B and transcription elongation factor B polypeptide 2.
• As part of the SIII/elongin complex, Elongin-B increases RNA polymerase II transcription elongation past template-encoded arresting sites. The transcriptional activity of subunit A is strongly enhanced by binding to the dimeric regulatory subunits B and C (elongin BC complex). Related references: PMID:7638163
• Elongin-B is a central component of ECS (Elongin-Cullin-SOCS box) ubiquitin ligase complexes, where it couples target-recruitment subunits (e.g., VHL, SOCS box proteins) to Cullin/RBX1 catalytic modules, mediating ubiquitination of diverse substrates. Related references: PMID:10205047 PMID:12004076 PMID:12050673
• Multiple ECS complexes containing Elongin-B participate in the DesCEND (destruction via C-end degrons) pathway, recognizing C-terminal degrons on target proteins to promote their ubiquitination and degradation. Related references: PMID:26138980 PMID:29775578 PMID:29779948
• Elongin-B-containing ECS complexes regulate key processes: ASB9 targets CKB for degradation (PMID:33268465), POLR2A is polyubiquitinated (PMID:19920177), RAB40C mediates ANKRD28 turnover to control cell migration (PMID:35512830), ASB7 degrades SUV39H1 to influence H3K9me3 marks (PMID:40440427), and LRRC58 targets CDO1 to modulate cysteine catabolism (PMID:40963025). Related references: PMID:33268465 PMID:19920177 PMID:35512830 PMID:40440427 PMID:40963025
• In HIV-1 infected cells, Elongin-B is hijacked by the viral Vif protein to form an ECS complex that ubiquitinates and degrades the antiviral cytidine deaminases APOBEC3F, APOBEC3G, and to some extent APOBEC3H, facilitating viral immune evasion. Related references: PMID:18562529 PMID:20532212 PMID:22190037 PMID:37640699
• Elongin-B is predominantly localized in the nucleus and undergoes post-translational modifications including acetylation and phosphorylation, consistent with its roles in transcription and ubiquitination regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to human TCEB2 amino acids 1–118; the antibody is expected to recognize full-length Elongin-B. For Western blotting, whole-cell lysates from human or mouse cell lines are suitable. Due to the small molecular weight (13 kDa), consider using 12–15% SDS-PAGE gels and extended transfer times with small-pore membranes (0.2 µm PVDF or nitrocellulose).
• In immunoprecipitation (IP), the polyclonal antibody may efficiently capture Elongin-B along with its interacting partners (e.g., Elongin-C, cullin-2/5, substrate receptors). Validate enrichment under non-denaturing conditions and confirm co-purification by mass spectrometry or Western blot.
• For ELISA, the rabbit polyclonal format provides broad epitope coverage; optimize coating conditions (antigen concentration, buffer, temperature) to ensure sensitivity and specificity in your assay system.
• As with all polyclonal antibodies, lot-to-lot consistency should be verified in your laboratory. We recommend titrating the antibody in your specific application and including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from concept to clinic. This anti-TCEB2/Elongin-B polyclonal antibody exemplifies our capability to supply critical reagents for transcription and ubiquitination pathway studies. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target sourcing to streamline your IVD development and research workflows.