TBX5 Rabbit Polyclonal Antibody for WB, ELISA - Q99593

Core Product Specifications and Parameters

Biological Background: TBX5 Function and Localization

• TBX5 is a T-box transcription factor encoded by the TBX5 gene (also known as HOS). Mutations in this gene cause Holt-Oram syndrome, characterized by congenital heart defects and upper limb abnormalities.
• It plays a critical role in heart development and limb pattern formation by regulating the transcription of downstream target genes. Related references: PMID:25725155, PMID:25963046, PMID:26917986
• TBX5 binds directly to the core DNA motif of the NPPA promoter, modulating cardiac gene expression. Related references: PMID:26926761
• The protein localizes to both the nucleus and the cytoplasm, with its primary function as a DNA-binding transcription factor in the nucleus.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing, producing multiple isoforms.
• Associated with atrial fibrillation and cardiomyopathy, TBX5 is listed under Uniprot keywords as a developmental protein and reference proteome component.
• The predicted molecular weight is approximately 57.7 kDa, consistent with the observed band at ~58 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (amino acids 1-70), which may offer specificity against other T-box family members—validate if cross-reactivity is a concern.
• For western blotting, expect a band around 58 kDa; consider using nuclear or whole-cell lysates from human, mouse, or rat tissue.
• In ELISA, performance can be confirmed with the recombinant immunogen or native protein from cell extracts; optimize coating conditions and antibody titration.
• No recommended dilution factors are provided; perform a series of dilutions to establish optimal working concentrations for your particular assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and researchers working on cardiac development and congenital disease targets. In addition to this TBX5 antibody, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to support your entire development pipeline—from concept to clinic.