TBX2 Rabbit pAb - Q13207

Core Product Specifications and Parameters

Biological Background: TBX2 Function and Localization

• Functions as a transcriptional repressor, binding to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' or half-sites to regulate gene expression. Related references: PMID:11062467, PMID:11111039, PMID:12000749
• Plays a critical role in embryonic development, including cardiac atrioventricular canal formation and limb patterning. Related references: PMID:29726930
• Promotes epithelial-mesenchymal transition (EMT) by repressing CDH1/E-cadherin expression, contributing to invasive and metastatic phenotypes. Related references: PMID:22844464
• Negatively regulates cellular senescence by targeting PML and CDKN1A/p21, and supports melanocyte proliferation. Related references: PMID:22002537
• Represses ADAM10 gene expression, possibly through HDAC1 recruitment, implicating it in Notch pathway modulation. Related references: PMID:30599067
• Localizes to the nucleus and is predominantly expressed in adult kidney, lung, and placenta, with weaker expression in heart and ovary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 604–712) of human TBX2, which is expected to be accessible in denatured western blot samples.
• For western blotting, TBX2 has a predicted molecular weight of ~75 kDa; confirm band migration in lysates from human, mouse, or rat origin.
• Polyclonal antibodies may exhibit batch-to-batch variation; titrate the antibody to optimize signal-to-noise ratio.
• If used in ELISA, the antibody can serve as a capture or detection reagent; complementary pairing and standard curves must be empirically established.
• Cross-reactivity with mouse and rat indicates potential for rodent model studies; validate specificity per species using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk reagents. This TBX2-targeting antibody supports research in developmental biology, EMT, and transcriptional regulation—key areas in oncology and genetic disorders. We help diagnostic manufacturers and labs accelerate assay development with reliable sourcing and technical support.