TBCA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75347

Core Product Specifications and Parameters

Biological Background: TBCA Function and Localization

• TBCA (Tubulin-specific chaperone A), also known as TCP1-chaperonin cofactor A or tubulin-folding cofactor A (CFA), is a key component of the tubulin folding pathway.
• It functions as a tubulin-folding protein, specifically involved in the early step of tubulin heterodimer assembly, ensuring proper α/β-tubulin folding and preventing non-productive interactions.
• The protein is predominantly localized to the cytoplasm and associates with the cytoskeleton, reflecting its role in microtubule dynamics.
• TBCA is subject to post-translational acetylation, which may modulate its chaperone activity or interaction with tubulin.
• Alternative splicing generates multiple isoforms, potentially expanding its functional repertoire.
• Keywords from UniProt include 3D-structure, Chaperone, Cytoskeleton, Microtubule, and Reference proteome, underscoring its established structural and functional characterization.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length TBCA protein (aa 1-108), suggesting broad epitope recognition; for WB, consider a standard denaturing protocol with a 13 kDa target and validate band specificity using positive and negative controls.
• For IHC-P, fix tissues appropriately and perform antigen retrieval as needed; a known TBCA-expressing tissue section (e.g., brain or testis) can serve as positive control, while pre-immune serum or blocking peptide helps confirm specificity.
• In ELISA, optimize coating antigen concentration and antibody dilution; check cross-reactivity with closely related chaperones if the target is part of a conserved family.
• Since TBCA is highly conserved across human, mouse, and rat, it is suitable for cross-species studies; always verify reactivity in your experimental system with a preliminary test.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including this polyclonal antibody against the tubulin chaperone TBCA. Our one-stop platform streamlines procurement of validated antibody pairs, bulk ancillary reagents, and custom monoclonal or polyclonal antibodies, extending to rare-target raw material sourcing for microtubule and cytoskeleton research. Let CamelBio bridge your project from concept to clinic with reliable, scalable supply solutions.