TAZ Rabbit Monoclonal Antibody for WB and ELISA - Q9GZV5

Core Product Specifications and Parameters

Biological Background: TAZ/WWTR1 Function and Localization

• WWTR1/TAZ is a transcriptional coactivator with a WW domain and PDZ-binding motif, playing a key role in the Hippo signaling pathway that controls organ size and suppresses tumors by limiting proliferation and promoting apoptosis. Related references: PMID:11118213, PMID:18227151, PMID:23911299

• In the Hippo kinase cascade, MST1/2 and LATS1/2 phosphorylate and inactivate TAZ, linking it to tumor suppression. Related references: PMID:18227151

• TAZ enhances the transcriptional activity of PAX8 and NKX2-1/TTF1, contributing to gene activation in specific tissues. Related references: PMID:19010321

• Together with YAP1, TAZ regulates TGFB1-dependent SMAD2/3 nuclear accumulation and couples SMADs to the mediator complex for transcription. Related references: PMID:18568018

• It promotes embryonic stem-cell self-renewal, cell proliferation, and epithelial-mesenchymal transition. Related references: PMID:18227151, PMID:18568018

• Subcellularly, TAZ localizes to the nucleus, cytoplasm, cell membrane, and tight junctions.

• Highly expressed in kidney, heart, placenta, lung, and thyroid tissue.

• Annotated as a proto-oncogene and phosphoprotein involved in transcription regulation, ubiquitin conjugation, and cell junction dynamics.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-270) of human TAZ, a well-conserved domain; for Western blotting, consider starting with a dilution range of 1:500–1:2000 and validate using lysates from human, mouse, or rat cell lines.
• For ELISA, the recombinant immunogen suggests suitability for both direct and capture formats; optimize coating and detection conditions with appropriate positive controls.
• When probing phosphorylated or ubiquitinated TAZ, pre-treat samples with phosphatase inhibitors or proteasome inhibitors as needed to preserve modification states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive portfolio of validated IVD raw materials, including carefully optimized monoclonal antibodies like this TAZ antibody, bulk ancillary reagents, and rare target sourcing support. With our one-stop supply model covering antibody pairs, recombinant proteins, and technical consultation, we help accelerate your assay development from concept to clinic. Explore our TAZ/Hippo pathway resources to streamline your cancer and stem cell research projects.