TARBP2 Rabbit pAb for WB - Q15633

Core Product Specifications and Parameters

Biological Background: TARBP2 Function and Localization

• TARBP2, also known as TRBP, is a RISC-loading complex subunit essential for RNA-induced silencing complex (RISC) formation. It operates within the RISC loading complex (RLC) alongside DICER1 and AGO2, processing precursor miRNAs (pre-miRNAs) to mature miRNAs and loading them onto AGO2 (PubMed:15973356, PubMed:16142218, PubMed:16271387).
  Related references: PMID:15973356, PMID:16142218, PMID:16271387

• The protein may also contribute to the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) via DICER1. For certain pre-miRNA substrates, TARBP2 can alter the cleavage site choice of DICER1 (PubMed:23063653).
  Related references: PMID:23063653

• TARBP2 negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting IRF7 phosphorylation and promoting its ubiquitination and degradation (PubMed:30927622).
  Related references: PMID:30927622

• During HIV-1 infection, TARBP2 binds to the viral TAR RNA, stimulating translation of TAR-containing RNAs. This occurs partly by inhibiting EIF2AK2/PKR, thereby reducing phosphorylation of EIF2S1/eIF-2-alpha (PubMed:11438532, PubMed:12475984, PubMed:2011739).
  Related references: PMID:11438532, PMID:12475984, PMID:2011739

• Additionally, TARBP2 mediates recruitment of the FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, which helps HIV-1 evade the innate immune system (PubMed:30626973).
  Related references: PMID:30626973

• Subcellularly, TARBP2 localizes to the cytoplasm, perinuclear region, and nucleus.

• The protein is classified under keywords including phosphoprotein, RNA-binding, translation regulation, and RNA-mediated gene silencing.

Experimental Guidance and Technical Tips

• The immunogen consists of full-length human TARBP2 (amino acids 1-366), which may provide broad recognition of native and recombinant protein forms.
• For Western blot (WB), an expected band near 39 kDa is consistent with the calculated molecular weight; validate in relevant cell lysates (consider human and mouse as per cross-reactivity data).
• For immunohistochemistry (IHC-P), standard antigen retrieval techniques and antibody titration are advisable; include appropriate tissue controls.
• In immunofluorescence (IF/ICC), permeabilization may be required to detect nuclear and perinuclear pools; fixation conditions should be optimized.
• ELISA applications may require coating antigen and antibody pair optimization; consider a direct or indirect format.
• As a polyclonal antibody, lot-to-lot consistency can be monitored by including a reference standard in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and laboratories with a comprehensive supply chain for IVD raw materials, including validated antibodies like this TARBP2 polyclonal antibody. Whether you need bulk quantities of optimized monoclonal/polyclonal antibodies, matched antibody pairs, or ancillary reagents for RNA silencing and viral infection research, CamelBio’s one-stop platform streamlines procurement from concept to clinic. We also specialize in sourcing rare-target raw materials, ensuring your projects advance without interruption.