TAF15 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q92804

Core Product Specifications and Parameters

Biological Background: TAF15 Function and Localization

• TAF15 is also known as TATA-binding protein-associated factor 2N (TAF2N), TAFII68 (68 kDa TATA-binding protein-associated factor), and RNA-binding protein 56 (RBP56).
• It functions as an RNA- and ssDNA-binding protein that plays specific roles during transcription initiation. It binds ssRNA containing the consensus sequence 5'-AGGUAA-3'. Related references: PMID:21256132
• TAF15 can enter the preinitiation complex together with RNA polymerase II (Pol II), contributing to promoter-specific transcription.
• The protein is predominantly localized in the nucleus but can also be detected in the cytoplasm.
• TAF15 is ubiquitously expressed in fetal and adult tissues.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, methylation, and ubiquitination, reflecting its dynamic regulation.
• It contains a zinc-finger domain and is implicated in chromosomal rearrangements linked to cancer, acting as a proto-oncogene.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 493–592 of human TAF15. The epitope likely resides in this C-terminal region.
• The antibody detects a band of approximately 62 kDa in Western blotting; consider using whole‑cell lysates from human, mouse, or rat cells.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody dilution in appropriate tissue samples.
• It is suitable for immunoprecipitation (IP) and ELISA, offering flexibility for various assay formats. Validate in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one‑stop partner for IVD raw‑material procurement, offering validated antibodies, optimized pairs, and bulk ancillary reagents to support diagnostic development. This TAF15 monoclonal antibody is part of our portfolio of transcription‑related research tools, suited for assay builders and core labs. Contact us for rare‑target sourcing, technical consultation, and seamless supply from concept to clinic.