T-bet/Tbx21 Rabbit mAb for WB, IHC-P, FC (intra), ELISA - Q9UL17

Core Product Specifications and Parameters

Biological Background: T-bet/Tbx21 Function and Localization

• Lineage-defining transcription factor that initiates Th1 lineage development from naive Th precursor cells by activating Th1 genetic programs and repressing the opposing Th2 and Th17 programs. Related references: PMID:10761931
• Activates transcription of signature Th1 genes such as IFNG and CXCR3 in part by recruiting chromatin remodeling complexes (KDM6B, SMARCA4-containing SWI/SNF, H3K4me2 methyltransferase) to establish permissive chromatin states. Related references: PMID:33296702
• Drives Th1 gene expression also via recruitment of Mediator complex and P-TEFb (super elongation complex) to super-enhancers and associated genes in activated Th1 cells. Related references: PMID:27292648
• Represses Th17 lineage commitment by blocking RUNX1-mediated transactivation of RORC, and suppresses Th2 cytokines (IL-4, IL-5, IL-13) through repression of GATA3 and NFATC2.
• Acts as a repressor of type-I IFN response in IFN-gamma abundant microenvironments, protecting Th1 cells from aberrant amplification.
• Required for correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells. Related references: PMID:33296702
• Regulates antiviral B-cell responses and promotes antiviral IgG2a isotype switching.
• Localizes to the nucleus and is expressed specifically in T-cells; subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 322-535) of human T-bet/Tbx21; consider that epitope recognition may be conformation-sensitive under certain denaturing conditions.
• For Western blot, validate in your sample system using reducing conditions; the expected molecular weight is ~58 kDa.
• For IHC-P, antigen retrieval conditions may require optimization; test both heat-induced citrate and EDTA buffers to achieve robust nuclear staining.
• For intracellular flow cytometry, ensure effective permeabilization and fixation protocols; use appropriate isotype controls to assess non-specific binding.
• Cross-reactivity is reported for human; reactivity with other species should be confirmed experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined, one-stop channel for high-quality IVD raw materials, including validated monoclonal antibodies like this anti-T-bet/Tbx21 rabbit mAb. For immune profiling and Th1/transcription factor research, we supply optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing, supporting your immunoassay development from concept to clinical application.