Syntaxin 4 Rabbit mAb - Q12846

Core Product Specifications and Parameters

Biological Background: Syntaxin-4 Function and Localization

• Syntaxin-4 (STX4), also known as renal carcinoma antigen NY-REN-31, is a member of the syntaxin family of SNARE proteins and functions as a plasma membrane t-SNARE.
• It mediates docking of transport vesicles and is essential for the translocation of SLC2A4 (GLUT4) from intracellular vesicles to the plasma membrane in response to insulin.
• In neurons, syntaxin-4 is recruited to neurite tip membrane domains rich in 1-oleoyl-2-palmitoyl-PC (OPPC), facilitating surface expression of the dopamine transporter SLC6A3/DAT.
• Syntaxin-4 is required for normal hearing, as evidenced by its role in docking/fusion events at stereocilia (PubMed:36355422). Related references: PMID:36355422
• Subcellularly, it localizes to the cell membrane, cell projections including neuron projections and stereocilia, and the perinuclear cytoplasm.
• It is expressed in neutrophils and neutrophil-differentiated HL-60 cells, with expression increasing upon differentiation.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword) and the presence of a coiled-coil domain and transmembrane helix; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band around 34 kDa; validate the antibody in the relevant tissue or cell lysate of human, mouse, or rat origin.
• In ELISA, optimize coating and detection conditions using appropriate controls, as performance may vary with sample type and protocol.
• When studying cross-reactivity with mouse or rat samples, confirm reactivity experimentally as sequence homology may influence detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For research and diagnostic development targeting syntaxin-4 in glucose metabolism, neuronal function, or hearing-related pathways, we provide streamlined access to rare target raw materials and technical consulting from concept to clinic.