Synaptophysin Rabbit mAb for WB, IHC, IF, IP, ELISA - P08247

Core Product Specifications and Parameters

Biological Background: Synaptophysin Function and Localization

• Synaptophysin, also known as major synaptic vesicle protein p38, is encoded by the SYP gene (Gene ID: 6855).
• It may play structural roles in organizing membrane components at synaptic vesicles and in targeting vesicles to the plasma membrane, and is involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
• The protein is located on the membrane of cytoplasmic secretory vesicles, specifically synaptic vesicles, and is enriched in synaptosomes.
• Predominantly expressed in the brain, with high levels in the hippocampus, the neuropil of the dentate gyrus (outer half of the molecular layer), CA4 and CA3 regions, and the putamen (at protein level). Related references: PMID:8838578 PMID:17296554
• Synaptophysin contains four transmembrane helices and has a calculated molecular weight of 33.8 kDa, migrating at ~34 kDa in SDS-PAGE.
• It is subject to alternative splicing, phosphorylation, ubiquitin conjugation, and glycosylation; keywords also include disease variant and intellectual disability, indicating potential clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 224-313 of human Synaptophysin, a C-terminal region likely accessible on the cytoplasmic side; consider its topology when designing detection protocols.
• For Western blotting, a specific band at ~34 kDa is expected in brain or synaptic vesicle-enriched lysates from human, mouse, or rat; validate with appropriate negative controls.
• In immunohistochemistry, robust staining is anticipated in brain tissue sections, particularly in the hippocampus and dentate gyrus; optimize antigen retrieval and antibody dilution for your sample type.
• For immunoprecipitation, use mild detergent lysis conditions to preserve protein complexes if studying synaptic vesicle interactions; include an isotype control.
• The antibody is suitable for multiple applications (WB, IP, IF, IHC, ELISA), but assay-specific optimization (e.g., fixation, blocking) is recommended to achieve optimal signal-to-noise.

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