Symmetric DiMethyl-Histone H4-R3 Rabbit Monoclonal Antibody (P62805) for Epigenetics Research

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is one of the core histones that constitute the eukaryotic nucleosome, around which DNA is wrapped to form chromatin. It plays a fundamental role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The dynamic post-translational modification of histones, often termed the "histone code," governs DNA accessibility to regulatory proteins and is central to epigenetic control. Related references: UniProt P62805

Histone H4 is localized primarily in the nucleus and associates with chromosomes. During spermatogenesis, acetylated H4 is localized to the nucleus in step 11 spermatids. Related references: UniProt P62805

Histone H4 undergoes extensive post-translational modifications, including acetylation, methylation, phosphorylation, and others. Symmetric dimethylation at Arg3 (SDMA-H4R3) is catalyzed by protein arginine methyltransferases PRMT5 and PRMT7 and is implicated in transcriptional regulation. Related references: UniProt P62805

Additional modifications include asymmetric dimethylation and citrullination at Arg3, acetylation at multiple lysine residues (e.g., K5, K8, K12, K16), and phosphorylation at specific serine and tyrosine residues. These combinatorial marks define distinct chromatin states. Related references: PMID:11387442 PMID:11448779 PMID:15345777 PMID:16567635 PMID:21724829 PMID:15592455 PMID:20068231

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal 1-100 amino acids of human Histone H4, which includes the Arg3 residue that is symmetrically dimethylated. When designing experiments, ensure that the antibody's specificity for this modification is verified using appropriate peptide competition or mutant controls.
• For Western Blot (WB), consider using nuclear extracts from human or rat cell lines. The predicted molecular weight is ~11 kDa. Validate the band size and specificity in your sample system.
• Immunofluorescence (IF/ICC) applications may require standard nuclear staining protocols. Co-staining with DNA dyes like DAPI can help confirm nuclear localization.
• Dot Blot (DB) and ELISA can be employed to quantify symmetric dimethyl-H4R3 levels in histone preparations or cell lysates. These assays are useful for high-throughput screening of methylation inhibitors or activators.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with reliable access to essential IVD raw materials, including this Symmetric DiMethyl-Histone H4-R3 Rabbit mAb for advanced epigenetic and chromatin research. Beyond specific antibodies, we provide a comprehensive range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services to accelerate your assay development from concept to clinic.