Symmetric DiMethyl-Histone H3-R8 Rabbit pAb (Q16695/P68431) for WB, IHC-P, IF/ICC, ELISA, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H3.1t / Histone H3.1 Function and Localization

Histone H3 variants (including H3.1t encoded by H3-4/HIST3H3 and H3.1 encoded by multiple gene clusters such as H3C1-H3C12) are core components of the nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility and playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.

Related references: UniProt P68431

• These histones are subject to a complex set of post-translational modifications (PTMs), collectively termed the histone code, which regulate chromatin dynamics and gene expression. Among these, symmetric dimethylation of arginine at position 8 (R8) in the mature histone H3 (corresponding to Arg9 in the UniProt sequence) is catalyzed by protein arginine methyltransferase 5 (PRMT5). This modification is associated with transcriptional repression and heterochromatin formation.

Related references: UniProt Q16695

• Histone H3.1t (UniProt Q16695) is a testis-specific variant expressed primarily in testicular cells, while H3.1 (UniProt P68431) represents the canonical somatic forms.
• Subcellular localization: Both variants are localized to the nucleus and chromosomes.

Experimental Guidance and Technical Tips

• Western Blot (WB): Expect a band at approximately 15 kDa. Use appropriate positive and negative controls (e.g., untreated vs. PRMT5 inhibitor-treated cells) to confirm symmetric dimethylation specificity. Consider peptide competition assays to validate antibody binding to the R8me2s epitope.
• Immunohistochemistry (IHC-P) and Immunofluorescence (IF/ICC): Optimize fixation and permeabilization steps, as epitope accessibility may vary. Since histone H3 is nuclear, ensure nuclear staining pattern and compare with total histone H3 or DAPI counterstaining.
• ELISA: This antibody can be used for direct or indirect ELISA to quantify symmetric dimethylation levels. Titrate antibody concentration and include appropriate blocking agents.
• ChIP-seq: Due to the high abundance of histone H3, optimize crosslinking and chromatin shearing. Use minimal input material and validate ChIP efficiency with positive and negative genomic loci (e.g., heterochromatic vs. euchromatic regions).
• General tip: Always include a non-modified peptide control to distinguish modification-specific signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined procurement pathway for IVD raw materials, from concept to clinic. This anti-symmetric dimethyl-histone H3-R8 antibody, targeting a critical epigenetic mark, is part of our comprehensive portfolio of methylated antibodies and histone modification reagents. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your IVD assay development and research programs. Partner with us to simplify your supply chain and advance your diagnostic projects.