Symmetric DiMethyl-Histone H3-R2 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, IHC, IF, ELISA, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is one of the core histone proteins, together with H2A, H2B, and H4, forming the nucleosome core particle. Nucleosomes wrap and compact DNA into chromatin, playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt Q16695, UniProt P68431

Both histone H3.1 (encoded by multiple genes including H3C1, H3C2, etc.) and H3.1t (encoded by H3-4) are expressed in human cells, with H3.1t predominantly found in testicular cells. Related references: UniProt Q16695

Histones undergo a vast array of post-translational modifications (PTMs), including acetylation, methylation, phosphorylation, citrullination, and more, collectively known as the "histone code". These modifications regulate chromatin structure and gene expression. Among these, symmetric dimethylation of arginine residues is catalyzed by protein arginine methyltransferases such as PRMT5. For example, symmetric dimethylation at histone H3 arginine 9 (H3R9me2s) has been reported. This antibody specifically recognizes symmetric dimethylated arginine 2 (H3R2me2s), a modification that may play a role in epigenetic regulation. Related references: UniProt Q16695

Experimental Guidance and Technical Tips

• The immunogen is a synthetic symmetric dimethylated peptide around R2, ensuring high specificity for the H3R2me2s modification. When performing Western blotting, histone-enriched nuclear extracts or acid-extracted histones may improve detection.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization conditions should be optimized for each tissue or cell type.
• In ChIP-seq experiments, consider including control reactions such as input DNA, IgG control, and possibly peptide competition to confirm binding specificity. Spike-in controls may be useful for quantitative normalization.
• ELISA-based approaches can be used for quantitative detection of H3R2me2s levels in cell lysates or purified histone preparations; ensure consistent protein loading and consider using a pan-H3 antibody for normalization.
• Cross-reactivity is reported for human, mouse, rat, and other species (wide range predicted). However, validation in your specific sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For epigenetic targets such as symmetric dimethyl-Histone H3-R2, CamelBio ensures access to rare and modification-specific antibodies, supporting your chromatin biology and diagnostic development projects. Our one-stop sourcing model streamlines your transition from concept to clinic.