SURF4 Rabbit pAb - O15260

Core Product Specifications and Parameters

Biological Background: SURF4 Function and Localization

• SURF4 (Surfeit locus protein 4), also known as ERV29, is an endoplasmic reticulum cargo receptor.
• SURF4 mediates the export of lipoproteins by recruiting cargos into COPII vesicles to facilitate their secretion. Related references: PMID:29643117, PMID:30251625, PMID:33186557
• It acts as a cargo receptor for lipoproteins bearing both APOB and APOA1, regulating lipoprotein delivery and lipid homeostasis. Related references: PMID:29643117, PMID:33186557
• SURF4 synergizes with the GTPase SAR1B to mediate transport of circulating lipoproteins. Related references: PMID:33186557
• It promotes the secretion of PCSK9. Related references: PMID:30251625
• SURF4 mediates the efficient secretion of erythropoietin (EPO). Related references: PMID:32989016
• It may also play a role in maintaining the architecture of the endoplasmic reticulum-Golgi intermediate compartment and the Golgi. Related references: PMID:18287528
• Subcellular localization: Endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane.
• Keywords: alternative splicing, protein transport, membrane protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50–150 of human SURF4 (NP_001267719.1). This region shows high homology between human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), the predicted molecular weight is ~30 kDa; ensure adequate separation and transfer conditions.
• Immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC) may require antigen retrieval and optimization of blocking conditions.
• ELISA can be used for quantitative detection or antibody titering; consider appropriate coating antigen and detection format.
• Validate antibody specificity in the relevant sample system (e.g., cell lines expressing SURF4) using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For lipid metabolism and lipoprotein secretion research, our portfolio includes polyclonal antibodies against SURF4 and related cargo receptors, alongside validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets.