SUMO2/3 Rabbit Polyclonal Antibody (WB, ELISA) - P61956 / P55854

Core Product Specifications and Parameters

Biological Background: SUMO2/3 Function and Localization

• SUMO2 (Small ubiquitin-related modifier 2) is a member of the SUMO protein family, which includes SUMO1, SUMO2, SUMO3, and SUMO4. SUMO2 and SUMO3 are highly similar (~97% identity) and often referred to collectively as SUMO2/3.
• SUMO2 functions as a ubiquitin-like protein that is covalently attached to lysine residues of target proteins, a process requiring E1 (SAE1-SAE2), E2 (UBE2I), and E3 ligases (e.g., PIAS1-4, RANBP2, CBX4, ZNF451). This modification regulates diverse cellular processes including nuclear transport, DNA replication and repair, mitosis, and signal transduction. Related references: PMID:26524494
• Polymeric SUMO2 chains can be polyubiquitinated, targeting the modified proteins for proteasomal degradation. Related references: PMID:18408734, PMID:18538659, PMID:21965678
• SUMO2 plays a role in regulating the sumoylation status of SETX, an RNA/DNA helicase involved in maintaining genomic stability. Related references: PMID:24105744
• Subcellular localization: SUMO2 is primarily localized in the nucleus, particularly in PML bodies, consistent with its role in nuclear processes.
• SUMO2 is broadly expressed across tissues, indicating its fundamental role in cellular homeostasis.
• Post-translational modifications include acetylation and sumoylation, which may regulate its activity and interactions.
• Keywords: SUMO2 is involved in host-virus interactions and is a component of the ubiquitin-like conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-95 amino acids of human SUMO2/3, which is conserved between SUMO2 and SUMO3. When using this antibody for Western blot (WB), validate in your specific sample system; free SUMO2/3 may be detected at ~11 kDa, while conjugated forms appear as higher molecular weight bands.
• For ELISA, optimization of antigen coating concentration and antibody dilution is recommended. Consider using a blocking buffer compatible with your assay conditions.
• Cross-reactivity with mouse and rat proteins has been reported, but we recommend confirming reactivity in your experimental model due to potential species-specific variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-SUMO2/3, and ancillary reagents. Our offering supports the study of ubiquitin-like modifications and proteasomal degradation pathways. We assist with sourcing rare targets and bulk reagent supply, helping you streamline from concept to clinical application.