STX1A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16623

Core Product Specifications and Parameters

Biological Background: Syntaxin-1A Function and Localization

• STX1A encodes syntaxin-1A, a neuron-specific membrane protein also known as HPC-1, which belongs to the syntaxin family of SNARE proteins and is essential for calcium-dependent exocytosis and endocytosis of hormones and neurotransmitters. Related references: PMID:26635000
• Syntaxin-1A forms the core SNARE complex together with SNAP25 on the plasma membrane and VAMP2 on synaptic vesicles; this complex drives synaptic vesicle fusion with the presynaptic membrane, enabling rapid neurotransmitter release.
• Beyond the central nervous system, STX1A participates in regulated exocytosis in other tissues, such as insulin secretion by pancreatic beta cells, GLP-1 release, and acrosomal exocytosis in sperm. Related references: PMID:23091057
• The protein localizes primarily to the cytoplasmic face of the plasma membrane and is enriched at synapses and synaptosomes; it is also found on secretory vesicles and can be secreted in a soluble form.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), which modulate SNARE complex assembly and stability, and the protein contains a coiled-coil domain critical for its function.
• STX1A is highly expressed in embryonic spinal cord, ganglia, adult cerebellum, and cerebral cortex, with additional expression in heart, liver, fat, skeletal muscle, and kidney.
• Alternative splicing generates multiple isoforms, and rare variants have been implicated in Williams-Beuren syndrome, highlighting the protein's clinical relevance in neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1–100 of human STX1A, which includes the N-terminal region; this region is relatively conserved between human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot, a band around 33 kDa is expected under reducing conditions; consider using brain or neuronal cell lysates as positive controls.
• In IHC-P and IF/ICC, antigen retrieval may be required, and formaldehyde fixation should be optimized; strong synaptic staining is anticipated in neural tissues.
• The antibody may be suitable for ELISA; however, the specific dilution factors should be determined empirically for each application and sample type.
• When interpreting results, note that STX1A exists in multiple compartments (plasma membrane, vesicles, secreted), so fractionation or co-localization studies may enrich specific pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic manufacturers and research laboratories by providing one-stop access to validated IVD raw materials, including this anti-STX1A polyclonal antibody. Whether you need bulk quantities of this antibody, complementary SNARE protein reagents, or customized assay development support, our integrated supply chain and technical team can help accelerate your neuroscience or metabolic disease projects from concept to clinic. Contact us to discuss your specific sourcing needs.