STOML2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UJZ1

Core Product Specifications and Parameters

Biological Background: STOML2 Function and Localization

• Stomatin-like protein 2 (STOML2), also known as SLP-2 or HSPC108, is a mitochondrial protein encoded by the STOML2 gene. It belongs to the stomatin family, sharing a conserved stomatin-like domain.
• STOML2 plays a key role in regulating mitochondrial biogenesis and activity. It stimulates cardiolipin biosynthesis and binds cardiolipin-enriched membranes, recruiting and stabilizing proteins like prohibitin to organize functional microdomains in mitochondrial membranes.
• Through modulation of mitochondrial function, STOML2 influences diverse biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis, and cellular stress responses.
• STOML2 negatively regulates calcium efflux from mitochondria, contributing to intracellular calcium homeostasis. It is also required for mitochondrial hyperfusion, a pro-survival stress response that enhances ATP production.
• At the cellular level, STOML2 localizes to multiple compartments: primarily the mitochondrion (inner membrane and intermembrane space), but also the cell membrane, membrane rafts, and cytoskeleton. This diverse localization suggests roles in both mitochondrial and plasma membrane organization.
• STOML2 may regulate T-cell activation by associating with the T-cell receptor signaling complex at the plasma membrane.
• Ubiquitously expressed at low levels, with confirmed protein expression in lymphoid tissues. This tissue distribution is consistent with its roles in immune cell function.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data. These modifications may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The recombinant immunogen (amino acids 29-356) covers a large region of human STOML2, suggesting the antibody may recognize multiple isoforms and is suitable for detecting endogenous STOML2 in human, mouse, and rat lysates.
• For WB, the calculated molecular weight based on the UniProt sequence is ~38.5 kDa; however, the supplier lists 39 kDa, which may reflect post-translational modifications or migration behavior. Consider running appropriate controls and using a prestained marker for accurate sizing.
• In IHC-P, antigen retrieval conditions should be optimized for the specific tissue and fixation method. Validate staining specificity using positive and negative control tissues or siRNA knockdown.
• The antibody's reactivity against mouse and rat STOML2 makes it useful for translational studies. Verify species-specific performance in your model system.
• Consider pairing this antibody with mitochondrial markers or cardiolipin probes to study STOML2's role in mitochondrial dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive procurement solution for IVD raw materials. Whether you need validated antibody pairs, bulk ancillary reagents, or specialized antibodies for rare targets like STOML2, CamelBio's extensive portfolio and expert sourcing bridge the gap from concept to clinic. Our one-stop platform accelerates development timelines for mitochondrial biology and immune function assays.