STIM1 Rabbit Monoclonal Antibody for WB, ELISA - Q13586

Core Product Specifications and Parameters

Biological Background: STIM1 Function and Localization

• STIM1 is a Ca2+ sensor that gates two major inward rectifying Ca2+ channels: CRAC channels and arachidonate-regulated Ca2+ (ARC) channels. Related references: PMID:15866891 PMID:16005298 PMID:16208375
• It mediates store-operated Ca2+ entry (SOCE) by translocating from the endoplasmic reticulum to the plasma membrane upon Ca2+ store depletion, activating CRAC channel pore-forming subunits ORAI1, ORAI2, and ORAI3. Related references: PMID:16208375 PMID:16537481 PMID:32415068
• STIM1 plays a role in enamel formation and regulates the type I interferon response by retaining STING at the endoplasmic reticulum. Related references: PMID:24621671 PMID:30643259
• The protein is ubiquitously expressed across various human primary cells and tumor cell lines.
• Subcellular localization includes the cell membrane, endoplasmic reticulum membrane, cytoplasm, cytoskeleton, and sarcoplasmic reticulum.
• As a glycoprotein and phosphoprotein, STIM1 undergoes post-translational modifications that may regulate its function.
• Alternative splicing produces multiple transcript variants, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 586–685 within the C‑terminal region of human STIM1. This region may be conserved across species, supporting the observed cross‑reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is approximately 77 kDa. Validate the antibody in your specific sample system, as post‑translational modifications or splice variants may alter observed migration.
• In ELISA applications, consider using the antibody as a capture or detection reagent for human, mouse, or rat STIM1, with appropriate controls.
• Since STIM1 functions in calcium signaling complexes, co‑immunoprecipitation or proximity‑based assays may be explored to study protein interactions, though such applications have not been validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw‑material procurement by providing a comprehensive portfolio of research‑grade antibodies, including this STIM1 monoclonal antibody for calcium signaling studies. As a dedicated sourcing partner, we connect diagnostic manufacturers and laboratories with validated antibody pairs, bulk ancillary reagents, and rare‑target raw materials, ensuring seamless project continuity from concept to clinic. Our expertise in IVD supply chain management bridges the gap between demanding research requirements and reliable, high‑quality raw material accessibility.