STEAP3 Rabbit Polyclonal Antibody for WB and ELISA - Q658P3

Core Product Specifications and Parameters

Biological Background: STEAP3 Function and Localization

• STEAP3, also known as Dudulin-2, TSAP6, or pHyde, is a six-transmembrane epithelial antigen of prostate 3 and a member of the STEAP family of metalloreductases.
• It functions as a NADPH-dependent ferric-chelate reductase, transferring electrons across the membrane to reduce Fe3+ chelates (PubMed:26205815). Related references: PMID:26205815
• STEAP3 can also reduce Cu2+ to Cu1+, indicating a broader role in metal ion homeostasis.
• It mediates efficient transferrin-dependent iron uptake in erythroid cells.
• Downstream of p53/TP53, STEAP3 participates in apoptosis and cell cycle regulation.
• STEAP3 is indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP (PubMed:15319436, PubMed:16651434). Related references: PMID:15319436 PMID:16651434
• Subcellularly, STEAP3 localizes to the endosome membrane.
• Tissue expression includes adult bone marrow, placenta, liver, skeletal muscle, and pancreas; it is down-regulated in hepatocellular carcinoma.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-180), which may be accessible in both native and denaturing conditions; consider standard blocking and detection protocols for Western blotting.
• Since the antibody recognizes human and mouse STEAP3, validate species-specific reactivity in your experimental model.
• For ELISA applications, inclusion of recombinant STEAP3 protein as a positive control is recommended.
• As STEAP3 is a multi-pass membrane protein, ensure adequate protein extraction and solubilization (e.g., using mild detergents) for optimal detection in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, including this high-quality STEAP3 antibody. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents to support diagnostic developers and researchers. Our supply chain ensures reliable access to rare-target antibodies and supporting proteins, streamlining your assay development from concept to clinic.