Staufen Rabbit Monoclonal Antibody for WB, ELISA - O95793

Core Product Specifications and Parameters

Biological Background: Staufen Function and Localization

• Staufen (STAU1) is a double-stranded RNA-binding protein encoded by the STAU1 gene, with multiple isoforms produced by alternative splicing.
• It binds dsRNA in a sequence-independent manner and also interacts with tubulin, suggesting a role in linking RNA transport to the cytoskeleton.
• Functions in the localization and translation of mRNAs at specific subcellular sites, possibly by anchoring RNA at cytoskeletal elements.
• In viral infections, Staufen aids in the production of virus particles for several viruses, including HIV-1, by interacting with viral budding proteins.
• Subcellularly, Staufen is found in the cytoplasm and associated with the rough endoplasmic reticulum.
• It is widely expressed, with notable presence in brain, pancreas, heart, skeletal muscle, liver, lung, kidney, and placenta.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 478-577 of human Staufen, covering a region that may be conserved across species, consistent with the reported cross-reactivity.
• For western blotting, a starting dilution range of 1:500–1:2000 is commonly used, with optimization recommended using a positive control lysate such as HeLa or HEK293 cells.
• For ELISA, consider using the immunizing peptide as a positive control and validate with a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, bulk ancillary reagents, and customized sourcing solutions. The Staufen rabbit monoclonal antibody is part of our high-quality product line, supporting researchers in RNA biology and virus-host interaction studies. From early-stage concept development to clinical readiness, CamelBio provides expert technical services and supply chain support to streamline your diagnostic projects.