STAT5A Rabbit mAb for WB, ELISA - P42229

Core Product Specifications and Parameters

Biological Background: STAT5A Function and Localization

• STAT5A (Signal Transducer and Activator of Transcription 5A) is a transcription factor that plays a critical role in cytokine and growth factor signaling.
• It mediates cellular responses to KITLG/SCF, ERBB4, and activated FGFRs, transducing signals from the cell surface to the nucleus.
• Upon activation, STAT5A binds to GAS elements in the promoters of target genes, regulating transcription of genes involved in cell proliferation, differentiation, and survival.
• In the cytoplasm, STAT5A is inactive; cytokine stimulation leads to phosphorylation and dimerization, followed by translocation to the nucleus where it acts as a DNA-binding transcriptional activator.
• It is essential for lactation, as it regulates the expression of milk protein genes in response to prolactin.
• STAT5A belongs to the STAT protein family and contains an SH2 domain critical for its recruitment to phosphorylated receptors and subsequent activation.
• Post-translational modifications, including phosphorylation and ubiquitination, modulate its transcriptional activity and stability.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human STAT5A. This region is not expected to interfere with the SH2 domain or DNA-binding domain, preserving antibody access to native epitopes.
• For western blotting (WB), a band of approximately 91 kDa is expected in human and mouse cell lysates. Consider using nuclear-enriched extracts to enhance detection, as STAT5A translocates to the nucleus upon activation.
• Due to high homology between human and mouse STAT5A, the antibody is reported to cross-react with mouse samples. Validate in the relevant tissue or cell line, such as mammary gland or immune cells.
• For ELISA applications, pair this monoclonal antibody with a suitable detection antibody recognizing a different epitope, and optimize coating and detection conditions.
• This product is provided as rabbit monoclonal antibody; thus, use appropriate anti-rabbit secondary antibodies for detection.
• Always include appropriate positive and negative controls, and refer to the provided shipping and storage instructions to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For STAT5A-related research, CamelBio offers validated antibody pairs, optimized monoclonal antibodies like this STAT5A Rabbit mAb, and bulk ancillary reagents for immunoassays. Whether you require rare target antibodies, technical consultation, or scalable sourcing solutions, CamelBio's one-stop platform streamlines your supply chain.