STAT5 Rabbit Monoclonal Antibody for WB and ELISA - P42229/P51692

Core Product Specifications and Parameters

Biological Background: STAT5 Function and Localization

• STAT5 (Signal Transducer and Activator of Transcription 5) comprises two closely related proteins, STAT5A and STAT5B, encoded by genes STAT5A (6776) and STAT5B (6777), which share high sequence homology and functional redundancy.
• STAT5A is a dual-function protein that mediates signal transduction and transcription activation in response to cytokines such as KITLG/SCF and growth factors including ERBB4 and FGFR family members.
• Upon receptor activation, STAT5A dimerizes, translocates to the nucleus, and binds to GAS (gamma-activated sequence) elements to regulate transcription of target genes, notably those involved in milk protein expression during lactation.
• In its inactive state, STAT5A is predominantly cytoplasmic; phosphorylation triggers its shuttling to the nucleus where it exerts its transcriptional regulatory functions.
• The protein contains an SH2 domain critical for receptor interaction and dimerization, and its activity is modulated by post-translational modifications including phosphorylation and ubiquitination (Ubl conjugation).
• STAT5A plays essential roles in lactation, immune cell development, and cellular proliferation, with tissue-specific functions in mammary gland, hematopoietic, and other tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 600-786) of human STAT5A, which encompasses part of the SH2 domain and the transactivation domain; high sequence conservation in this region likely confers cross-reactivity with STAT5B.
• For Western blot, a band around 90 kDa is expected; consider using STAT5 knockout or siRNA-treated lysates to confirm band specificity.
• In ELISA applications, pairing with a capture antibody targeting a non-competing epitope may improve assay performance; titrate both antibodies for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with seamless access to this STAT5 monoclonal antibody and a full spectrum of IVD raw materials. Whether you require validated antibody pairs for ELISA development, bulk quantities of monoclonal antibodies, or specialized sourcing for STAT family targets, CamelBio’s integrated platform supports your project from concept to clinic. With reliable supply and expert technical guidance, we empower your research in cytokine signaling, transcriptional regulation, and lactation biology.