STAT3 Rabbit pAb for WB, IF/ICC, ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• STAT3 mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors by acting as a signal transducer and transcription activator. Related references: PMID:10688651 PMID:11294841 PMID:12359225

• Upon activation, STAT3 recruits coactivators like NCOA1 or MED1 to promoters and binds to IL6-responsive elements, regulating acute-phase protein genes. Related references: PMID:15653507 PMID:16285960 PMID:17344214 PMID:12359225

• STAT3 is a key regulator of inflammatory and immune responses, controlling naive CD4+ T-cell differentiation into Th17 or regulatory T-cells; acetylation promotes this activity. Related references: PMID:28065600 PMID:28262505

• It promotes cell cycle progression by inducing CCND1 expression for G1/S transition. Related references: PMID:17344214

• STAT3 also influences apoptosis through BIRC5 transactivation and represses macroautophagy by inhibiting EIF2AK2/PKR. Related references: PMID:18242580 PMID:23084476

• Subcellularly, STAT3 localizes to both cytoplasm and nucleus, shuttling upon activation.

• STAT3 is expressed across a wide range of tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, and in various T-cell subsets. Related references: PMID:31899195

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 88 kDa; consider using appropriate positive controls (e.g., HeLa or A431 cell lysates) and validate band specificity.
• In IF/ICC, anticipate nuclear/cytoplasmic localization upon stimulation; optimize fixation and permeabilization conditions for your cell system.
• For ELISA, evaluate optimal antigen coating concentrations and antibody dilutions; the immunogen corresponds to the C-terminal region (aa 600-700), which may be suitable for capture or detection formats.
• Cross-reactivity is shown with human, mouse, and rat STAT3; verify in your species and sample model of interest.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibodies, antigen pairs, and ancillary reagents. This STAT3 antibody fits into signal transduction, cancer, and immunology research applications. We support diagnostic manufacturers with bulk orders, custom development, and technical consulting from concept to clinic.