STAT1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 is a member of the signal transducer and activator of transcription (STAT) protein family; two isoforms exist (alpha and beta) via alternative splicing.
• Functions as a key mediator of interferon, cytokine, and growth factor signaling, coupling receptor activation to gene expression. Related references: PMID:12764129, PMID:12855578, PMID:15322115, PMID:15526160, PMID:19088846
• In type I IFN signaling, phosphorylated STAT1 forms ISGF3 complex with STAT2/IRF9 to bind ISRE; type II IFN induces STAT1 homodimer (GAF) binding GAS, driving antiviral transcription. Related references: PMID:28753426, PMID:35568036, PMID:8156998
• Post-translationally regulated by phosphorylation: Tyr-701 and Ser-727 are critical for activation; Thr-749 phosphorylation by IKBKB regulates ARID5A and IL12B under LPS/TLR4 signaling. Related references: PMID:26479788, PMID:32209697
• Predominantly cytoplasmic in resting cells; translocates to nucleus upon activation.
• In the small intestine, STAT1 cooperates with Gasdermin-D p13 to induce CIITA, promoting food tolerance via Tr1 cell differentiation (By similarity).
• Mutations in STAT1 are linked to immunodeficiency disorders (e.g., IMD31, CANDF7) and susceptibility to viral/mycobacterial infections, consistent with its antiviral roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the C-terminal region (aa 650-750) of human STAT1, contributing to specificity for both STAT1 alpha (91 kDa) and beta (84 kDa) isoforms.
• For WB, the predicted molecular weight is ~87 kDa; both alpha and beta isoforms may be observed depending on sample and conditions.
• In IHC-P and IF/ICC, optimize fixation, permeabilization, and antibody dilution using positive control tissues or cells known to express STAT1.
• This polyclonal antibody is suitable for IP and ELISA; perform titration experiments to determine optimal working concentrations for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide high-quality IVD raw materials, including this STAT1 antibody for interferon signaling, immunology, and infectious disease research. Our offerings span validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing for rare targets — helping you accelerate development from concept to clinic. Contact us to discuss your procurement needs and discover reliable supply solutions.