ST3GAL3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q11203

Core Product Specifications and Parameters

Biological Background: ST3GAL3 Function and Localization

• ST3GAL3 encodes CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase (Alpha 2,3-ST 3), a member of the glycosyltransferase family 29 that catalyzes the transfer of sialic acid to terminal galactose residues on glycoproteins and glycolipids.
• The enzyme preferentially sialylates Gal-beta-1,3-GlcNAc over Gal-beta-1,3-GalNAc, generating key NeuAc-alpha-2,3-Gal linkages essential for cell–cell recognition, adhesion, and signaling.
• ST3GAL3 is a type II transmembrane protein localized predominantly to the Golgi stack membrane, where the majority of terminal glycosylation occurs; a soluble form can also be secreted.
• Tissue expression is highest in adult skeletal muscle and throughout fetal development, with lower levels in placenta, lung, and liver, implying developmental and tissue-specific functional requirements.
• The protein bears a signal-anchor transmembrane domain, an intramolecular disulfide bond, and is itself glycosylated; alternative splicing produces multiple isoforms.
• Genetic variants in ST3GAL3 are associated with an autosomal recessive form of intellectual disability and epileptic encephalopathy, underscoring its role in neural development and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–375, covering the entire catalytic domain, which supports recognition of endogenous ST3GAL3 in diverse applications; consider using positive controls such as skeletal muscle or fetal tissue lysates.
• For western blotting, a band near 42 kDa is expected; due to glycosylation and potential cleavage/secretion, additional higher or lower molecular-weight species may be observed—validate in the relevant sample system.
• In IHC-P, incorporate an antigen retrieval step (e.g., citrate buffer, pH 6.0) and test both membranous (Golgi) and diffuse cytoplasmic staining patterns.
• For IF/ICC, permeabilization is required to detect Golgi-resident ST3GAL3; co-staining with a Golgi marker is recommended to confirm localization.
• The antibody is predicted to react with human, mouse, and rat; cross-reactivity in other species should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials. For glycosylation-related research and assay development, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti‑ST3GAL3 reagent, bulk ancillary reagents, and offer rare‑target raw‑material sourcing. Whether you need single reagents or full workflow support, our team is ready to assist from concept to clinic.