SSH2 Rabbit Polyclonal Antibody for WB and ELISA - Q76I76

Core Product Specifications and Parameters

Biological Background: SSH2 Function and Localization

• SSH2, also known as SSH-2L or hSSH-2L, is a protein phosphatase encoded by the SSH2 gene (GeneID: 85464) in humans.
• It regulates actin filament dynamics by dephosphorylating and activating cofilin, which promotes actin depolymerization; it also inactivates LIMK1 through dephosphorylation. Related references: PMID:11832213
• SSH2 is required for spermatogenesis and is involved in acrosome biogenesis, likely via cofilin-mediated actin remodeling during vesicle fusion and trafficking.
• The protein localizes to the cytoplasm, cytoskeleton, focal adhesions, and acrosomal vesicles.
• It contains actin-binding domains and undergoes alternative splicing, producing multiple isoforms.
• SSH2 is subject to phosphorylation and is classified as a phosphoprotein and hydrolase.
• Its molecular weight is approximately 158 kDa, consistent with the observed protein weight in assays.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 158 kDa; use appropriate positive and negative controls to validate specificity.
• In ELISA, optimize coating conditions and antibody dilutions to achieve optimal signal-to-noise ratios.
• The antibody cross-reacts with human, mouse, and rat, enabling multispecies studies; confirm cross-reactivity in your experimental system.
• Consider peptide blocking or knockdown/overexpression experiments to verify antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, supporting the entire workflow from concept to clinic. This anti-SSH2 polyclonal antibody serves research in actin cytoskeleton dynamics, cell motility, and spermatogenesis. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials for diagnostic development.