SRSF10 Rabbit pAb for WB and ELISA - O75494

Core Product Specifications and Parameters

Biological Background: SRSF10 Function and Localization

• SRSF10, also known as SRp38, SRrp40, and FUSIP1, is a serine/arginine-rich splicing factor that belongs to the SR protein family and plays a dual role in constitutive and alternative pre-mRNA splicing.
• In its dephosphorylated form, SRSF10 acts as a general repressor of pre-mRNA splicing by interfering with the U1 snRNP 5'-splice site recognition of SNRNP70; this activity is crucial for splicing repression during M-phase and after heat shock.
  Related references:
  PMID:11684676 PMID:12419250 PMID:14765198
• SRSF10 specifically promotes exon skipping during alternative splicing; interaction with YTHDC1, which recognizes m6A-containing RNAs, prevents SRSF10 from binding near m6A sites and thus inhibits exon skipping.
  Related references:
  PMID:26876937
• Alternative splicing of SRSF10 generates isoforms with distinct regulatory functions; isoform 1 acts as a positive regulator while isoform 3 acts as a negative regulator of neuronal alternative splicing.
  Related references:
  PMID:12419250
• Subcellularly, SRSF10 localizes to nuclear speckles—dynamic structures for pre-mRNA processing—and to the cytoplasm, consistent with its shuttling role in mRNA metabolism.
• The protein is widely expressed across tissues and is phosphorylated under normal conditions; dephosphorylation activates its repressor function, highlighting the importance of post-translational control. (Keywords: Phosphoprotein)
• UniProt keywords for SRSF10 include: Alternative splicing, mRNA processing, mRNA splicing, Nucleus, Cytoplasm, Phosphoprotein, RNA-binding.

Experimental Guidance and Technical Tips

• The immunogen used for this rabbit polyclonal antibody is a synthetic peptide spanning residues 163-262 of human SRSF10. Consider using blocking peptide competition to confirm specificity in your assays.
• For Western blotting, start with a dilution range of 1:500–1:2000 and validate in lysates from human, mouse, or rat cell lines expressing SRSF10. The predicted molecular weight is approximately 31 kDa; ensure appropriate gel percentage for resolution.
• For ELISA, optimize coating antigen and antibody dilutions using standard protocols. The antibody may detect both phosphorylated and unphosphorylated forms; include phosphatase inhibitors in lysis buffers if studying phosphorylation-dependent activities.
• As SRSF10 function is tightly linked to stress responses, experimental conditions (e.g., heat shock, cell cycle synchronization) can significantly influence its abundance and phosphorylation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialize in rare target raw-material procurement. Whether you are developing splicing-focused diagnostic assays or exploring SRSF10's role in disease, our SRSF10 antibodies and customized raw-material solutions accelerate your research and product development.