SREK1 Rabbit pAb - Q8WXA9

Core Product Specifications and Parameters

Biological Background: SREK1 Function and Localization

• SREK1, also known as SFRS12, SRrp86, or SRrp508, is a splicing regulatory glutamine/lysine-rich protein identified as a component of the spliceosome and involved in alternative splicing regulation.
• The protein functions as a modulator of splicing factor activity: it inhibits the splicing activity of SFRS1 (ASF/SF2), SFRS2 (SC35), and SFRS6 (SRp55), while augmenting the splicing activity of SFRS3 (SRp20) (By similarity).
• SREK1 belongs to the serine/arginine-rich (SR) protein family, which are key regulators of constitutive and alternative pre-mRNA splicing, influencing splice site selection.
• Subcellular localization is primarily in the nucleus, consistent with its role in mRNA processing and spliceosomal dynamics.
• Post-translational modifications include phosphorylation (as indicated by the keyword 'Phosphoprotein') and ubiquitination ('Ubl conjugation'), which may regulate its activity and interactions.
• The protein contains an isopeptide bond, suggesting potential structural complexity or linkage with other proteins, although its functional significance remains to be fully elucidated.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody corresponds to the N-terminal 180 amino acids of human SREK1. When performing western blotting, ensure thorough protein extraction from nuclear fractions to effectively detect this nuclear-localized target.
• For ELISA applications, consider using recombinant SREK1 protein fragments as coating antigens and validate the antibody's specificity against related splicing factors to control for cross-reactivity.
• Given the polyclonal nature and cross-reactivity with mouse SREK1, the antibody may recognize multiple isoforms; verify expected bands and species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing services. Serving as a dedicated sourcing bridge, CamelBio supports every stage from concept to clinic, ensuring efficient procurement of high-quality reagents for advanced research needs—whether you are investigating splicing regulatory mechanisms or developing novel diagnostic assays.