SPRED1 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z699

Core Product Specifications and Parameters

Biological Background: SPRED1 Function and Localization

• SPRED1 (Sprouty-related, EVH1 domain-containing protein 1) is a tyrosine kinase substrate that negatively regulates growth factor-mediated MAP kinase activation.
• It inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation by suppressing ERK1/2 phosphorylation.
• SPRED1 negatively regulates hematopoiesis of bone marrow and inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells.
• The protein attenuates actin stress fiber formation by inhibiting TESK1-mediated cofilin phosphorylation. Related references: PMID:18216281
• Subcellularly, SPRED1 localizes to the cell membrane, caveolae, and nucleus.
• Weak tissue expression has been observed in embryonic HEK293 cells. Post-translational modifications include acetylation, methylation, phosphorylation, palmitoylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 120-320 of human SPRED1 (NP_689807.1); for Western blot, an expected band around 50 kDa can be observed in relevant cell lysates such as HEK293.
• For ELISA applications, consider using the recombinant protein as a standard to establish assay conditions.
• Since this is a polyclonal antibody, validate batch-to-batch consistency and specificity in the experimental system of interest.
• Cross-reactivity with mouse SPRED1 suggests potential utility in mouse model studies, but independent validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supplies reliable antibodies like this anti-SPRED1 polyclonal for cell signaling research, alongside validated antibody pairs, optimized monoclonal options, and bulk ancillary reagents tailored to your assay development. Whether you need rare target sourcing or scaled-up production, our one-stop supply chain bridges your concept to clinic with consistent quality and technical support.