SPIRE2 Rabbit Polyclonal Antibody for WB and ELISA - Q8WWL2

Core Product Specifications and Parameters

Biological Background: SPIRE2 Function and Localization

• SPIRE2 (Spir-2) is an actin nucleation factor that remains associated with the slow-growing pointed end of actin filaments, directly regulating actin dynamics. Related references: PMID:21620703
• It provides a functional link between actin cytoskeleton reorganization and intracellular vesicle transport along actin fibers.
• SPIRE2 is required for asymmetric spindle positioning and asymmetric cell division during meiosis, ensuring proper chromosome segregation. Related references: PMID:21620703
• The protein is necessary for normal formation of the cleavage furrow and polar body extrusion in female germ cell meiosis. Related references: PMID:21620703
• In the nucleus, SPIRE2 cooperates with SPIRE1 to promote assembly of nuclear actin filaments in response to DNA damage, facilitating chromatin movement and repair factor dynamics. Related references: PMID:26287480
• Subcellular localization includes cytoplasm, cytoskeleton, cytosol, cell membrane, and cytoplasmic vesicle membrane.
• The protein is classified under keywords: actin-binding, alternative splicing, phosphoprotein, protein transport, repeat, reference proteome.
• SPIRE2 has a predicted molecular weight of ~80 kDa (79.7 kDa) and belongs to the spire family of actin nucleation factors.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–190) of human SPIRE2; consider sequence alignment to assess epitope conservation in mouse and rat.
• For Western blotting, expect a band near 80 kDa; validate specificity by including positive and negative control lysates (e.g., SPIRE2 knockdown or overexpressing lines).
• In ELISA, titrate the antibody carefully and include the immunogen recombinant protein as a standard to evaluate assay performance.
• As a polyclonal antibody, it may recognize multiple isoforms or degradation products; use appropriate loading controls and replicate experiments to confirm results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research teams. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For projects involving SPIRE2 or related cytoskeletal targets, we offer tailored sourcing support from concept to clinic.