SP4 Rabbit Polyclonal Antibody for WB and ELISA - Q02446

Core Product Specifications and Parameters

Biological Background: SP4 Function and Localization

• SP4 (Specificity protein 4) is a member of the Sp transcription factor family, characterized by three C2H2-type zinc finger motifs at the C-terminus that mediate DNA binding. It is also known as SPR-1.
• SP4 functions as a transcriptional activator by binding to GC- and GT-box promoter elements, regulating the expression of target genes.
• SP4 is highly expressed in the brain, suggesting a critical role in neural gene regulation and development.
• The protein localizes to the nucleus, consistent with its role as a transcription factor.
• SP4 undergoes phosphorylation, as indicated by phosphoproteomic data, which may modulate its transcriptional activity and stability.
• Keywords from UniProt highlight its DNA-binding, metal-binding (zinc), and transcription regulation properties, and it is part of the Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280-440 of human SP4 (NP_003103.2), covering part of the N-terminal region and central domain before the zinc fingers. This region may be less conserved, providing specificity.
• For WB, consider a dilution range of 1:500-1:2000 and validate in lysates from brain tissues or cell lines expressing SP4 (e.g., neuronal cell lines). A band of approximately 82 kDa is expected.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and validate with recombinant SP4 protein.
• Since SP4 is a transcription factor expressed predominantly in the nucleus, nuclear extraction protocols may enhance detection by WB.
• Cross-reactivity with mouse and rat SP4 is reported, but always verify sequence homology and confirm in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This SP4 antibody supports studies in transcriptional regulation and neuroscience, offering reliable performance for WB and ELISA. In addition to this polyclonal antibody, CamelBio can supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for your IVD development needs, including assistance with rare target raw-material sourcing.