SP140 Rabbit pAb for WB and ELISA - Q13342

Core Product Specifications and Parameters

Biological Background: SP140 Function and Localization

• SP140 is a component of nuclear bodies (also known as PML bodies, nuclear domain 10, or KR bodies) and may be involved in the pathogenesis of acute promyelocytic leukemia and viral infection. Related references:
  PMID:8910577
• It belongs to the SP100 family and is also referred to as lymphoid-restricted homolog of Sp100 (LYSp100), nuclear autoantigen Sp-140, and speckled 140 kDa protein.
• SP140 may play a role in chromatin-mediated regulation of gene expression, although it does not bind to histone H3 tails directly. Related references:
  PMID:24267382
• The protein localizes primarily to the nucleus, specifically to PML bodies, but is also found in the cytoplasm.
• SP140 is highly expressed in spleen and peripheral blood leukocytes, with lower levels in tonsils, thymus, prostate, ovary, small intestine, and colon, and very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Related references:
  PMID:8695863
  PMID:8910577
• Contains a bromodomain and zinc-finger motifs, suggesting roles in DNA binding and chromatin interaction.
• Post-translationally modified by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-180) of human SP140, which may contribute to specific detection of the full-length protein.
• For Western blotting, consider using spleen or leukocyte lysates where SP140 is highly expressed; the predicted molecular weight is ~98 kDa.
• Cross-reactivity with mouse and rat is indicated; validate these species' samples before use.
• For ELISA, optimize antibody dilution and include appropriate controls (e.g., recombinant SP140 or cell extracts).

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CamelBio is your one-stop sourcing bridge for IVD raw materials, connecting you with high-quality antibodies like this SP140 polyclonal antibody for nuclear body and chromatin research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare targets to support your diagnostic development from concept to clinic.