SOX9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P48436

Core Product Specifications and Parameters

Biological Background: SOX9 Function and Localization

• Transcription factor SOX-9 is a master regulator of chondrogenesis and skeletal development, binding the 5'-ACAAAG-3' DNA motif to activate cartilage-specific genes like COL2A1, ACAN, SOX5, and SOX6. Related references: PMID:8640233
• It drives multiple stages of chondrocyte differentiation: precartilaginous condensation, overt chondrogenesis (requiring SOX5 and SOX6), and hypertrophic maturation while suppressing osteoblast differentiation by inhibiting β-catenin/RUNX2 signaling.
• SOX-9 also functions in epithelial stem/progenitor cells, regulating branching morphogenesis in lung and kidney development through balancing proliferation and extracellular matrix dynamics.
• Localized exclusively to the nucleus, SOX-9 acts as a transcriptional activator, with its activity modulated by post-translational modifications including acetylation, phosphorylation, and ubiquitination.
• Low lipid levels promote chondrogenic commitment via FOXO transcription factors, which induce SOX9 expression to suppress fatty acid oxidation and favor chondrogenesis. Related references: PMID:24038782
• Mutations in SOX9 are linked to campomelic dysplasia (CMD1) and 46,XY sex reversal (SRXY10), underscoring its essential roles in development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250-339 of human SOX9, a region conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For western blotting, expect a band at approximately 56 kDa; consider using nuclear extracts and titrating the antibody to reduce background.
• In IHC-P, antigen retrieval (e.g., citrate buffer) and validation on cartilage or testis sections may enhance staining; specific staining should localize to the nucleus.
• For ELISA, this antibody can be used for antigen detection; optimize coating and detection conditions based on your assay platform.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material supply solutions, connecting diagnostic manufacturers and laboratories with high-quality reagents. This SOX9 Rabbit Polyclonal Antibody supports research into skeletal development and chondrogenesis. Beyond individual antibodies, we offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your procurement from concept to clinic.