SOX2 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P48431

Core Product Specifications and Parameters

Biological Background: SOX2 Function and Localization

• SOX2 is a transcription factor essential for embryonic development and maintenance of embryonic stem cell pluripotency. It forms a trimeric complex with OCT4 on DNA to regulate genes such as YES1, FGF4, UTF1, and ZFP206. Related references:
  PMID:18035408
• SOX2 binds to the proximal enhancer region of NANOG, a key pluripotency factor, and is critical for early embryogenesis.
• In neural stem cells, SOX2 promotes self-renewal and maintains an undifferentiated state by counteracting proneural proteins and suppressing neuronal differentiation.
• The protein localizes primarily to the nucleus and nuclear speckles, but has also been detected in the cytoplasm.
• SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors and contains a DNA-binding HMG domain.
• Post-translational modifications include phosphorylation, methylation, and ubiquitination, which may regulate its activity and stability.
• Disease-associated variants in SOX2 are linked to microphthalmia and other developmental eye defects.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-190) of human SOX2, so the antibody likely targets this domain; epitope specificity should be validated if used for domain-specific studies.
• For Western blot (WB), use nuclear-enriched lysates and expect a band at ~34 kDa; antibody dilution and blocking conditions should be empirically optimized.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; consider testing citrate or EDTA-based protocols.
• For immunofluorescence (IF/ICC), fixation and permeabilization methods (e.g., 4% PFA with 0.1% Triton X-100) can affect staining; include appropriate controls.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to reserve a reference batch for long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive supply chain for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes. This SOX2 rabbit polyclonal antibody is ideal for stem cell and developmental biology applications, and we offer bulk quantities, validated antibody pairs, and complementary reagents such as recombinant proteins and detection systems. For specialized or rare-target sourcing needs, our team ensures efficient procurement from concept to clinic.