SOX11 Rabbit pAb for WB, IF/ICC, ELISA - P35716

Core Product Specifications and Parameters

Biological Background: SOX11 Function and Localization

• SOX-11 functions as a transcriptional activator, driving the expression of genes essential for development (PubMed:24886874, PubMed:26543203). Related references: PMID:24886874 PMID:26543203
• It cooperates with POU-domain transcription factors, such as POU3F2/BRN2 and POU3F1/OCT6, to enhance transcriptional activation.
• SOX-11 directly activates TEAD2 expression by binding to its promoter and first intron.
• Along with SOX4 and SOX12, SOX-11 promotes cell survival in developing tissues including the neural tube, branchial arches, and somites, contributing to organogenesis.
• The protein resides in the nucleus, consistent with its DNA-binding transcription factor role.
• SOX-11 is predominantly expressed in the brain and heart, with lower expression in kidney, pancreas, and muscle.
• It undergoes phosphorylation, and structural studies have provided insights into its DNA-binding mechanism.
• Mutations in SOX11 are associated with intellectual disability, highlighting its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100); consider validating specificity for full-length and potential isoforms in your system.
• For Western blotting, a band near 47 kDa (theoretical 46.7 kDa) is expected; optimize antibody dilution and blocking conditions.
• In immunofluorescence/immunocytochemistry, anticipate nuclear staining; include appropriate controls to confirm localization.
• For ELISA, titrate antibody concentration and verify binding to the immunogen peptide or full-length protein.
• Cross-reactivity with mouse and rat suggests utility across rodent models; confirm performance in target tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality antibodies like this SOX11 polyclonal antibody, supporting transcription factor research and neurodevelopmental studies. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. We streamline procurement from concept to clinic, offering technical services and consulting to accelerate your IVD development.