SOX10 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P56693

Core Product Specifications and Parameters

Biological Background: SOX10 Function and Localization

• SOX10 (SRY-box transcription factor 10) is a member of the SOX family of transcription factors, crucial for development and differentiation of neural crest-derived cells. It is also known by aliases DOM, WS4, PCWH, WS2E, WS4C.
• SOX10 activates expression of myelin genes (e.g., DUSP15, MYRF, MBP) and plays a central role in oligodendrocyte maturation and CNS myelination.
• It acts as a transcriptional activator of MITF, synergizing with PAX3. Related references: PMID:21965087
• SOX10 localizes to the cytoplasm, nucleus, and mitochondrial outer membrane, indicating diverse regulatory roles.
• It is expressed in fetal brain and in adult brain, heart, small intestine, and colon, underscoring its broad tissue relevance.
• The protein undergoes alternative splicing and phosphorylation, and is associated with several diseases including Waardenburg syndrome, Hirschsprung disease, and Kallmann syndrome.
• SOX10 functions as a DNA-binding transcriptional activator, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; validate performance in your specific assay system and sample type.
• The immunogen corresponds to amino acids 270-360 of human SOX10 (C-terminal region). For cross-reactivity with mouse and rat, confirm sequence homology in this region.
• For immunohistochemistry (IHC-P), consider appropriate antigen retrieval methods and antibody titration based on your tissue of interest.
• In Western blot (WB), the predicted molecular weight of SOX10 is approximately 50 kDa; however, post-translational modifications may shift electrophoretic mobility.
• When using for immunofluorescence (IF/ICC), include proper fixation and permeabilization steps to detect nuclear, cytoplasmic, or mitochondrial localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material supplier, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing services to support diagnostic and research applications. This anti-SOX10 polyclonal antibody, targeting a key transcription factor in myelination and neural crest development, exemplifies our commitment to providing high-quality reagents for neuroscience and oncology research. Whether you need ready-to-use antibodies, customized production, or technical consultation, CamelBio is your trusted sourcing partner from concept to clinic.