SOX10 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P56693

Core Product Specifications and Parameters

Biological Background: SOX10 Function and Localization

• SOX10 is a transcription factor essential for the development and maturation of glial cells, including oligodendrocytes (By similarity).
• It specifically activates myelin genes like DUSP15 and MYRF to promote CNS myelination, and cooperates with MYRF in the myelination program (By similarity).
• SOX10 transcriptionally activates MITF, acting synergistically with PAX3 (PubMed:21965087). Related references: PMID:21965087
• The protein shuttles between the cytoplasm, nucleus, and mitochondrial outer membrane.
• It is expressed in fetal brain and adult brain, heart, small intestine, and colon.
• SOX10 is associated with Waardenburg syndrome, Hirschsprung disease, Kallmann syndrome, and deafness; it undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 270-360 in the C-terminal region of human SOX10, which may provide specificity to the antibody. Consider confirming reactivity with endogenous protein in appropriate cell or tissue lysates.
• For western blotting (WB), a band around 50 kDa is expected; validate with positive controls expressing SOX10 and consider using nuclear or whole-cell extracts.
• For immunohistochemistry (IHC-P), standard antigen retrieval techniques may be required; verify staining specificity using known SOX10-expressing tissues such as brain or glial tumors.
• For immunofluorescence/immunocytochemistry (IF/ICC), note that SOX10 can localize to both the nucleus and cytoplasm; include appropriate organelle markers and DAPI counterstaining.
• ELISA performance should be assessed with the specific assay format; the recombinant protein used as immunogen may serve as a positive control for coating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials. This SOX10 antibody supports neurological research and diagnostic development targeting glial cell transcription factors. Beyond individual antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—partner with CamelBio to streamline your entire assay development workflow from concept to clinic.