SOD3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08294

Core Product Specifications and Parameters

Biological Background: SOD3 Function and Localization

• Also known as extracellular superoxide dismutase [Cu-Zn] (EC-SOD), SOD3 is a secreted antioxidant enzyme that protects the extracellular space from oxidative damage by catalyzing the dismutation of superoxide radicals into hydrogen peroxide and oxygen.
• It functions as a homotetramer that binds copper and zinc ions as essential cofactors, critical for its enzymatic activity and structural stability.
• The protein contains an N-terminal signal peptide directing it to the secretory pathway, a central catalytic domain with Cu/Zn binding sites, and a C-terminal heparin-binding domain that mediates interaction with extracellular matrix components.
• SOD3 is localized to the extracellular space, including the extracellular matrix and on cell surfaces, but is also found in the Golgi apparatus and trans-Golgi network during secretion.
• Tissue expression is prominent in blood vessels, heart, lung, kidney, and placenta, where it represents the major SOD isoenzyme in extracellular fluids such as plasma, lymph, and synovial fluid.
• Post-translational modifications include formation of an intramolecular disulfide bond, N-linked glycosylation, and susceptibility to glycation, which may modulate its activity and stability.
• Keywords: 3D-structure, Antioxidant, Copper, Direct protein sequencing, Disulfide bond, Glycation, Glycoprotein, Golgi apparatus, Heparin-binding, Metal-binding, Oxidoreductase, Proteomics identification, Reference proteome, Secreted, Signal, Zinc.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19–160 of human SOD3, which spans the mature N-terminal region and part of the catalytic domain; consider this when assessing isoform specificity.
• For western blotting, under reducing conditions anticipate a band near 26–30 kDa; the secreted nature may require concentration of cell culture supernatants or bodily fluids for detection.
• In immunohistochemistry (paraffin) and immunofluorescence, evaluate whether antigen retrieval and permeabilization steps are needed, as the target is predominantly extracellular and may be lost without careful fixation.
• For ELISA applications, this polyclonal antibody may be used as capture or detection reagent; pairing with a compatible monoclonal antibody could enhance assay sensitivity.
• Cross-reactivity with mouse and rat SOD3 is indicated, but verify performance in your specific model system, especially if sequence homology varies in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to a comprehensive portfolio of IVD raw materials and technical support. For oxidative stress and antioxidant research targeting SOD3, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in obtaining rare target raw materials. Our one-stop service model streamlines your journey from concept to clinic, helping you secure the critical reagents needed for robust assay development.