SNX4 Rabbit Monoclonal Antibody for WB, IP, ELISA - O95219

Core Product Specifications and Parameters

Biological Background: SNX4 Function and Localization

• SNX4, or sorting nexin-4, is a member of the sorting nexin family of proteins, which are characterized by the presence of a phox-homology (PX) domain that facilitates phosphoinositide binding and membrane association.
• SNX4 plays a central role in regulating endocytosis and multiple stages of intracellular trafficking (PubMed:12668730, PubMed:17994011, PubMed:32513819). Related references: PMID:12668730 PMID:17994011 PMID:32513819
• It is specifically involved in the recycling of endocytosed transferrin receptor, preventing its lysosomal degradation and thus maintaining iron homeostasis (PubMed:17994011). Related references: PMID:17994011
• SNX4 contributes to autophagosome biogenesis by mediating the trafficking and recycling of the phospholipid scramblase ATG9A, a key protein required for autophagy (PubMed:32513819, PubMed:33468622). Related references: PMID:32513819 PMID:33468622
• Subcellularly, SNX4 localizes predominantly to the cytoplasmic face of early endosome membranes, where it participates in cargo sorting and vesicle formation.
• Post-translational modifications of SNX4 include acetylation and phosphorylation, which may regulate its activity, localization, or protein interactions.
• The SNX4 gene undergoes alternative splicing, giving rise to multiple transcript variants that may encode distinct protein isoforms with potentially different functions.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to a recombinant fragment covering amino acids 321–400 of human SNX4, near the C-terminal region, which may allow recognition of native and denatured protein conformations.
• For Western blotting, prepare whole-cell lysates using standard RIPA or SDS sample buffer; a protein band near 52 kDa is expected under reducing conditions. Consider including a positive control such as HeLa or HEK293T cell extracts, which express endogenous SNX4.
• In immunoprecipitation assays, optimize the antibody-to-lysate ratio and use a compatible bead system (e.g., protein A/G agarose) for rabbit IgGs. Validate recovery by Western blot with a different anti-SNX4 antibody.
• For ELISA applications, test a range of coating antigen concentrations and antibody dilutions using recombinant SNX4 protein or peptide. Ensure the epitope is accessible in the coating format.
• The antibody cross-reacts with mouse SNX4; perform initial validation in both human and mouse model systems to confirm species-specific performance.
• Because SNX4 functions in dynamic membrane trafficking pathways, fixation and permeabilization conditions for immunofluorescence may require optimization, though this application has not been specified for this product.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw material and services partner, connecting diagnostic manufacturers and researchers with critical reagents from concept to clinic. This SNX4 rabbit monoclonal antibody supports investigations into endocytosis, autophagy, and intracellular trafficking—areas central to neurodegeneration, cancer, and infectious disease research. Beyond individual antibodies, CamelBio can source validated antibody pairs, optimized monoclonal and polyclonal reagents, ancillary bulk materials, and even rare-target raw materials to streamline your entire assay development pipeline.