SNX18 Rabbit Polyclonal Antibody for WB and ELISA - Q96RF0

Core Product Specifications and Parameters

Biological Background: SNX18 Function and Localization

• SNX18, also designated SH3 and PX domain-containing protein 3B (SH3PXD3B) and SNAG1, is a sorting nexin family member containing both a phox homology (PX) lipid-binding domain and an SH3 domain.
• It is involved in endocytosis and intracellular vesicle trafficking during interphase and mitosis, playing roles in both clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis as well as macropinocytosis. Related references: PMID:18411244, PMID:20427313, PMID:21048941
• SNX18 binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate, promotes membrane tubulation, stimulates the GTPase activity of dynamin-2 (DNM2), and facilitates DNM2 localization at the plasma membrane. Related references: PMID:18411244, PMID:20427313
• It is required for efficient mitosis and cytokinesis, specifically for normal cleavage furrow formation. Related references: PMID:22718350
• Together with DNM2, SNX18 regulates autophagosome assembly by controlling trafficking of the phospholipid scramblase ATG9A from recycling endosomes. Related references: PMID:29437695
• Subcellularly, SNX18 localizes predominantly to endosome membranes, recycling endosomes, the cell membrane, and cytoplasmic vesicles.
• The UniProt record associates SNX18 with keywords such as Alternative splicing, Cell cycle, Cell division, Endocytosis, Lipid-binding, Membrane, Mitosis, Protein transport, and SH3 domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 445-624 of human SNX18, a region that may present conformational epitopes; for ELISA, consider using the recombinant fragment as a coating antigen alongside purified full-length protein.
• For Western blot, the predicted molecular weight is approximately 69 kDa; however, post-translational modifications or alternative splicing may result in additional bands. Validate specificity using appropriate positive and negative controls in your sample system.
• Given the polyclonal nature, lot-to-lot variation may occur; include an internal reference standard when performing quantitative comparisons.
• Cross-reactivity with mouse and rat suggests conserved epitopes; confirm in the relevant species by sequence alignment or experimental testing if needed.
• When studying membrane-associated SNX18, consider detergent-based lysis buffers to ensure efficient solubilization of the target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing a comprehensive portfolio of antibodies, reagents, and technical support for diagnostic manufacturers and research laboratories. This SNX18 polyclonal antibody exemplifies our commitment to supplying validated antibodies for membrane trafficking and cell division research. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material sourcing to streamline your assay development from concept to clinic.