SNRPD2 Rabbit pAb for WB, IHC-P, ELISA - P62316

Core Product Specifications and Parameters

Biological Background: SNRPD2 Function and Localization

• SNRPD2 (Sm-D2) is a core protein component of the spliceosomal U1, U2, U4, and U5 small nuclear ribonucleoproteins (snRNPs), the fundamental building blocks of the spliceosome.
  Related references:
  PMID:11991638
  PMID:18984161
  PMID:19325628

• The protein is integral to both the pre-catalytic spliceosome B complex and the activated C complex, playing a dynamic role in splicing catalysis.
  Related references:
  PMID:11991638
  PMID:28076346
  PMID:28502770

• As a component of the minor spliceosome, SNRPD2 is involved in the removal of U12-type introns, a specialized class of pre-mRNA introns.
  Related references:
  PMID:15146077

• Subcellularly, SNRPD2 localizes to both the cytoplasm (cytosol) and the nucleus, reflecting its roles in snRNP assembly and nuclear splicing activity.

• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its function and interactions. Alternative splicing gives rise to multiple isoforms.

• The protein has a predicted molecular weight of approximately 13.5 kDa (observed at 14 kDa) and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 1–118 of human SNRPD2, covering the N-terminal region; the antibody is expected to detect full-length endogenous SNRPD2 across species.
• For Western blot, a 14 kDa band is anticipated; include positive control lysates from human, mouse, or rat cell lines expressing SNRPD2.
• For immunohistochemistry (paraffin), consider appropriate antigen retrieval and validate staining patterns with known tissue distribution.
• While ELISA applications are possible, optimization of coating conditions and detection systems is advised.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated provider of IVD raw materials, CamelBio connects diagnostic manufacturers and research labs with high-quality antibodies and ancillary reagents. This anti-SNRPD2 polyclonal antibody supports splicing research and pre-mRNA processing studies. Beyond individual antibodies, CamelBio offers validated pairs, bulk reagents, and customized sourcing to accelerate your projects from concept to clinic.