SNRPA1 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - P09661

Core Product Specifications and Parameters

Biological Background: SNRPA1 Function and Localization

• SNRPA1 (also known as Lea1, U2A') is the U2 small nuclear ribonucleoprotein A', a core component of the U2 snRNP particle and the spliceosome, essential for pre-mRNA splicing. Related references: PMID:11991638, PMID:27035939, PMID:28076346
• Within the spliceosome, SNRPA1 specifically contributes to the binding and recognition of stem loop IV of the U2 small nuclear RNA (snRNA), a critical step in spliceosomal assembly. Related references: PMID:27035939, PMID:32494006, PMID:9716128
• The protein localizes strictly to the nucleus, consistent with its role in mRNA processing and splicing.
• SNRPA1 contains leucine-rich repeats (LRRs), a structural motif likely involved in RNA-binding and protein-protein interactions within the U2 snRNP.
• Post-translational modifications of SNRPA1 include acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond), which may regulate its splicing activity or complex stability.
• The calculated molecular weight of SNRPA1 is approximately 28.4 kDa; the recombinant antibody detects a band at 28 kDa under standard conditions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 156–255 of human SNRPA1 (P09661). Peptide competition or blocking assays may be considered to confirm binding specificity.
• Validated applications include Western blot (WB), immunoprecipitation (IP), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. Performance should be verified in the end user’s specific sample and protocol.
• Based on sequence homology, cross-reactivity is predicted with human, mouse, and rat samples. Validate detection in each species under your experimental conditions.
• Optimal antibody dilutions and incubation times should be determined empirically, as they depend on sample type, target abundance, and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored for splicing research and U2 snRNP targets. For SNRPA1 and related spliceosome components, we offer reliable sourcing solutions to accelerate your assay development and production needs.