SNAP91 Rabbit Polyclonal Antibody for WB, ELISA - O60641

Core Product Specifications and Parameters

Biological Background: SNAP91/AP180 Function and Localization

• SNAP91 encodes clathrin coat assembly protein AP180, also known as 91 kDa synaptosomal-associated protein, CALM, and AP180.
• AP180 functions as an adaptin component linking clathrin to receptors in coated vesicles, and its binding to clathrin triskelia promotes assembly into 60-70 nm coats.
• The protein localizes primarily to the cell membrane and coated pits.
• Post-translational modifications include glycosylation, methylation, and phosphorylation, as indicated by UniProt keywords.
• The gene undergoes alternative splicing, resulting in multiple isoforms (RefSeq NP_001229723.1 corresponds to the immunogen region).
• AP180 is involved in protein transport, particularly in clathrin-mediated endocytosis.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated using a recombinant fragment corresponding to amino acids 321-600, which lies within the central region of AP180 and may detect multiple isoforms.
• The antibody has been validated for WB and ELISA; cross-reactivity with mouse and rat is reported, making it suitable for rodent model studies.
• For WB, the predicted molecular weight is 93 kDa; however, post-translational modifications might result in slight shifts.
• Consider optimizing blocking and washing conditions to minimize background, as is typical for polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with integrated access to IVD raw materials, including validated antibodies like this anti-SNAP91 rabbit pAb for clathrin-mediated endocytosis studies. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare target sourcing, CamelBio offers a seamless procurement experience to support your workflow from concept to clinic.