SNAP25 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P60880

Core Product Specifications and Parameters

Biological Background: SNAP25 Function and Localization

• SNAP25 (Synaptosomal-associated protein 25) is a 25 kDa t-SNARE protein also known as SUP, RIC4, SEC9, and SNAP-25.
• Functions as a t-SNARE involved in the molecular regulation of neurotransmitter release by mediating vesicle docking and membrane fusion at the synapse.
• Associates with proteins involved in synaptic vesicle exocytosis and regulates plasma membrane recycling through its interaction with CENPF.
• Modulates the gating characteristics of the voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
• Subcellularly located in the cytoplasm (perinuclear region), cell membrane, synapse, synaptosome, and photoreceptor inner segment.
• Specifically expressed in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
• Undergoes post-translational modifications including palmitoylation and phosphorylation; disease variants are associated with epilepsy and intellectual disability (Keywords: alternative splicing, coiled coil, lipoprotein, phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-206), which may help detect full-length and some truncated forms; consider validating specificity in knockout or knockdown systems.
• For Western blot, an expected band around 23 kDa should be observed; use appropriate loading controls and confirm molecular weight markers.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization protocols; signal may be enriched at synaptic sites.
• Cross-reactivity with mouse and rat allows application in rodent models; verify specificity in the intended species/tissue.
• For ELISA, consider pairing with a suitable capture or detection antibody; test optimal concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, supporting every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This SNAP25 antibody supports neuroscience and synaptic function research, and we offer comprehensive procurement solutions for your development pipeline.