SMYD4 Rabbit Polyclonal Antibody for WB - Q8IYR2

Core Product Specifications and Parameters

Biological Background: SMYD4 Function and Localization

• SMYD4 (SET and MYND domain-containing protein 4) is a protein-lysine N-methyltransferase that catalyzes the transfer of methyl groups to lysine residues, utilizing S-adenosyl-L-methionine as a cofactor.
• It monomethylates PRMT5, a key factor in transcriptional regulation, thereby modulating its activity. Related references: PMID:38438251
• SMYD4 may also function as a histone methyltransferase, potentially influencing chromatin structure and gene expression (By similarity).
• Plays an essential role in cardiac development, acting as an epigenetic regulator of gene expression through its dual activities as a methyltransferase and negative regulator of HDAC1 (By similarity).
• The protein contains characteristic SET and MYND zinc-finger domains, classifying it within the SMYD family of methyltransferases.
• It localizes to both the nucleus and the cytoplasm, suggesting dynamic shuttling or context-dependent functions.
• Keywords associated with SMYD4 include metal-binding, zinc-finger, methyltransferase, and nucleus, highlighting its structural and enzymatic features.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 260 amino acids of human SMYD4; verify the epitope recognition in your target species if sequences are not fully conserved.
• For Western blot, a band corresponding to the predicted molecular weight of ~89 kDa may be observed; consider using appropriate gel percentages and transfer conditions for larger proteins.
• In immunofluorescence or immunohistochemistry, expect both nuclear and cytoplasmic staining based on the known subcellular localization of SMYD4; include suitable controls to confirm specificity.
• This polyclonal antibody may be suitable for immunoprecipitation due to its ability to recognize multiple epitopes; validate IP conditions in the relevant sample system.
• The reported cross-reactivity with mouse and rat suggests that the immunogen sequence is well conserved; however, always confirm reactivity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable access to IVD raw materials, including this rabbit polyclonal SMYD4 antibody for epigenetic and cardiac research. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your assay development from concept to clinic. For specialized or rare target raw-material sourcing, CamelBio serves as your dedicated procurement bridge.