SMYD4 Rabbit pAb - Q8IYR2

Core Product Specifications and Parameters

Biological Background: SMYD4 Function and Localization

• SMYD4 is a protein-lysine N-methyltransferase that monomethylates PRMT5, thereby modulating its transcriptional activity. Related references: PMID:38438251
• It may also function as a histone methyltransferase (by similarity).
• SMYD4 contains SET and MYND domains, characteristic of this family of methyltransferases.
• It plays a critical role in cardiac development by acting as an epigenetic regulator, negatively regulating HDAC1 in addition to its methyltransferase activity (by similarity).
• Localizes to both the nucleus and cytoplasm.
• Involved in metal-binding and zinc-finger-mediated interactions, consistent with its zinc-finger domains.

Experimental Guidance and Technical Tips

• For western blotting, expect a band around 89–90 kDa. Validate detection in relevant sample systems.
• In immunofluorescence/ICC, anticipate nuclear and cytoplasmic staining patterns. Use appropriate fixation and permeabilization methods.
• For immunoprecipitation, this antibody may enrich full-length SMYD4. Confirm complex formation under native conditions.
• As a polyclonal antibody, batch-to-batch variability should be considered; include positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials for cardiac and epigenetic research, including this anti-SMYD4 antibody. Our portfolio encompasses validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. We support diagnostic manufacturers and labs from concept to clinic with reliable supply solutions.