SMYD1 Rabbit pAb - Q8NB12

Core Product Specifications and Parameters

Biological Background: SMYD1 Function and Localization

• SMYD1 (SET and MYND domain-containing protein 1) is a histone-lysine N-methyltransferase.
• Methylates histone H3 at 'Lys-4' (H3K4), capable of mono-, di-, and trimethylation, and acts as a transcriptional repressor.
• Essential for cardiomyocyte differentiation and cardiac morphogenesis.
• Contains SET and MYND zinc finger domains, suggesting roles in DNA-binding and protein interactions.
• Subcellular localization includes both cytoplasm and nucleus.
• Tissue expression appears mostly restricted to heart and skeletal muscle.
• Classified under keywords such as Methyltransferase, Transferase, Zinc-finger, and Repressor; requires S-adenosyl-L-methionine as cofactor.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-350 of human SMYD1) may confer specificity to the N-terminal region; validate in relevant sample systems.
• Given strong expression in heart and skeletal muscle, consider using tissue lysates from these sources for WB or ELISA.
• Predicted protein weight of ~57 kDa; optimize gel percentage and transfer conditions accordingly.
• Cross-reactivity with mouse and rat SMYD1 enables use in common animal models; verify reactivity in your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw material sourcing partner for diagnostic manufacturers, research labs, and institutes. For cardiac and muscle development studies involving SMYD1, we can facilitate access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our team also supports rare target raw-material sourcing, helping you accelerate assay development from concept to clinic.